Inbred corn line ZSO1602

ABSTRACT

Broadly this invention provides inbred corn line ZS01602. The methods for producing a corn plant by crossing the inbred line ZS01602 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01602 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01602 with at least one other corn line.

FIELD OF THE INVENTION

This invention is in the field of corn breeding, specifically relatingto an inbred corn line designated ZS01602.

BACKGROUND OF THE INVENTION

The original maize plant was indigenous to the Western Hemisphere. Theplants were weedlike and only through the efforts of early breeders wasa cultivated crop species developed. The physical traits of maize aresuch that self pollination or cross pollination can occur. Each planthas a separate male and female flower, the tassel and ear, respectively.Natural pollination occurs when wind transfers pollen from tassel to thesilks on the corn ears. This type of pollination contributed to the widevariation of maize varieties present in the Western Hemisphere.

The development of a planned breeding program for maize only occurred inthe last century. Originally, maize was an open pollinated varietyhaving heterogeneous genotypes. The maize farmer selected uniform earsfrom the yield of these genotypes and reserved them for planting thenext season. The result was a field of maize plants that weresegregating for a variety of traits. This type of maize selection leadto at most incremental increases in seed yield.

Large increases in seed yield were the result of the development ofhybrid corn varieties in planned breeding programs. Hybrids weredeveloped by selecting corn lines and selfing these lines for severalgenerations to develop homozygous pure inbred lines and crossingselected inbred lines with unrelated inbred lines to produce hybridprogeny (F1). Inbred lines can be difficult to produce since theinbreeding process in corn decreases the vigor. However, when two inbredlines are crossed, the hybrid plant evidences greatly increased vigorcompared to open pollinated segregating maize plants. An importantfactor of the homozygosity and the homogeneity of the inbred lines isthat the hybrid from any cross will always be the same, and can bereproduced.

The ultimate objective of the commercial maize seed companies is toproduce high yielding, agronomically sound plants which perform well incertain regions or areas of the Corn Belt. To produce these types ofhybrids, the companies must develop inbreds which carry needed traitsinto the hybrid combination. Hybrids are not uniformly adapted for theCorn Belt, but are specifically adapted for regions of the Corn Belt.Northern regions of the Corn Belt require shorter season hybrids than dosouthern regions of the Corn Belt. Hybrids that grow well in Coloradoand Nebraska soils may not flourish in rich Illinois soil. Thus, avariety of major agronomic traits are important in hybrid combinationfor the various Corn Belt regions, and have an impact on hybridperformance.

Inbred line development and hybrid testing have been emphasized in thepast half century in commercial maize production as a means to increasehybrid performance. Inbred development is usually done by pedigreeselection. Pedigree selection can be selection in an F₂ populationproduced from a planned cross of two genotypes (often elite inbredlines), or selection of progeny of synthetic varieties, open pollinated,composite, or backcross populations. This type of selection is effectivefor highly inheritable traits, but other traits, for example, yieldrequires replicated test crosses at a variety of stages for accurateselection.

Maize breeders select for a variety of traits in inbreds that impacthybrid performance along with selecting for acceptable parental traits.Such traits include yield potential in hybrid combination; dry down;maturity; grain moisture at harvest; greensnap; resistance to rootlodging; resistance to stalk lodging; grain quality; disease and insectresistance; ear and plant height; performance in different soil typessuch as: low level of organic matter, clay, sand, black, high pH, lowpH; performance in: wet environments, drought environments, and notillage conditions. These traits appear to be governed by a complexgenetic system that makes selection and breeding of an inbred lineextremely difficult. Even if an inbred in hybrid combination hasexcellent yield (a desired characteristic), it may not be useful becauseit fails to have acceptable parental traits such as seed yield, seedsize, pollen production, good silks, plant height, etc.

To illustrate the difficulty of breeding and developing inbred lines,the following example is given. Two inbreds compared for similarity of29 traits differed significantly for 18 traits between the two lines. If18 simply inherited single gene traits were polymorphic with genefrequencies of 0.5 in the parental lines, and assuming independentsegregation (as would essentially be the case if each trait resided on adifferent chromosome arm), then the specific combination of these traitsas embodied in an inbred would only be expected to become fixed at arate of one in 262,144 possible homozygous genetic combinations.Selection of the specific inbred combination is also influenced by thespecific selection environment on many of these 18 traits which makesthe probability of obtaining this one inbred even more remote. Thus, thegeneral procedure of producing a non segregating F₁ generation and selfpollinating to produce a F₂ generation that segregates for traits doesnot easily lead to a useful inbred. Great care and breeder expertisemust be used in selection of breeding material to continue to increaseyield and agronomics of inbreds and resultant commercial hybrids.

SUMMARY OF THE INVENTION

The present invention relates to an inbred corn line ZS01602.Specifically, this invention relates to plants and seeds of this line.Additionally, this relates to a method of producing hybrid seed cornfrom this inbred. More particularly, this invention relates to theunique combination of traits that combine in corn line ZS01602.

Generally then, broadly the present invention includes an inbred cornseed designated ZS01602. This seed produces a corn plant.

The invention also includes the tissue culture of regenerable cells ofZS01602 wherein the tissue regenerates plants having the genotype ofZS01602. The tissue culture is selected from the group consisting ofleaves, pollen, embryos, roots, root tips, anthers, silk, flowers,kernels, ears, cobs, husks and stalks, and cells and protoplaststhereof. The corn plant regenerated from ZS01602 having ZS01602'sgenotype.

The invention extends to hybrid seed produced by planting, inpollinating proximity, seeds of corn inbred lines ZS01602 and anotherinbred line; cultivating corn plants resulting from said planting;preventing pollen production by the plants of one of the inbred lines;allowing natural cross pollinating to occur between said inbred lines;and harvesting seeds produced on plants of the inbred. The hybrid seedproduced by hybrid combination of plants of inbred corn seed designatedZS01602 and plants of another inbred line. Hybrid plants grown from thishybrid seed.

The invention further includes a method of hybrid F1 production. A firstgeneration (F1) hybrid corn plant produced by the process of planting,in pollinating proximity, seeds of corn inbred lines ZS01602 and anotherinbred line; cultivating corn plants resulting from said planting;preventing pollen production by the plants of one of the inbred lines;allowing natural cross pollinating to occur between said inbred lines;harvesting seeds produced on plants of the inbred; and growing aharvested seed.

A tissue culture of the regenerable cells of hybrid plants produced withuse of ZS01602 genetic material. A tissue culture of the regenerablecells of the corn plant produced by the method described above.

DEFINITIONS

In the description and examples which follow, a number of terms areused. In order to provide a clear and consistent understanding of thespecifications and claims, including the scope to be given such terms,the following definitions are provided.

BL MOIST

The moisture percentage of the grain at black layer, i.e., when 50% ofthe plants per plot have reached physiological maturity.

COLD GERM

Cold Germ is a measurement of seed germination under cold soilconditions. Data is reported as percent of seed germinating.

ECB

European corn borer a maize eating insect. ECBI is the first broodgeneration of European corn borers. ECBII is the second generation ofEuropean corn borers.

EMERGE

The number of emerged plants per plot (planted at the same seedlingrate) collected when plants have two fully developed leaves.

GI

This is a selection index which provides a single quantitative measureof the worth of a hybrid based on four traits. Yield is the primarytrait contributing to index values. The GI value is calculated bycombining stalk lodging, root lodging, yield and dropped ears accordingto the attached mathematical formula:

    GI=100+0.5(YLD)-0.09(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR)

GLS

Gray Leaf Spot (Cercospora zeae) disease rating. This is rated on a 1-9scale with a "1" being very susceptible, and a "9" being veryresistant.*

GW

Goss' Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scalewith a "1" being very susceptible, and a "9" being very resistant.*

HEATP10

The number of Growing Degree Units (GDU's) or heat units required for aninbred line or hybrid to have approximately 10 percent of the plantsshedding pollen. This trait is measured from the time of planting.Growing Degree Units are calculated by the Barger Method where the GDU'sfor a 24 hour period are: ##EQU1## The highest maximum temperature usedis 86° F. and the lowest minimum temperature used is 50° F. For eachinbred or hybrid it takes a certain number of GDU's to reach variousstages of plant development.

HEATBL

The number of GDU's after planting when approximately 50 percent of theinbred or hybrid plants in a plot have grain which has reachedphysiological maturity (black layer).

HEATPEEK

The number of GDU's after planting of an inbred when approximately 50percent of the plants show visible tassel extension.

HEATP50 or HTP50

The number of GDU's required for an inbred or hybrid to haveapproximately 50 percent of the plants shedding pollen. Growing DegreeUnits are calculated by the Barger Method as shown in the HEATP10definition.

HEATP90

The number of GDU's accumulated from planting when the last 100 percentof plants in an inbred or hybrid are still shedding enough viable pollenfor pollination to occur. Growing Degree Units are calculated by theBarger Method as shown in the HEATP10 definition.

HEATS10

The number of GDU's required for an inbred or hybrid to haveapproximately 10 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

HEATS50 or HTS50

The number of GDU's required for an inbred or hybrid to haveapproximately 50 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

HEATS90

The number of GDU's required for an inbred or hybrid to haveapproximately 90 percent of the plants with silk emergence of at least0.5 inches. Growing Degree Units are calculated by the Barger Method asshown in the HEATP10 definition.

MDMV_(A)

Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale witha "1" being very susceptible, and a "9" being very resistant.*

MDMV_(B)

Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a"1" being very susceptible and a "9" being very resistant.*

MOISTURE

The average percentage grain moisture of an inbred or hybrid at harvesttime.

NLB

Northern Leaf Blight (Exserohilum turcicum) disease rating. This israted on a 1-9 scale with a "1" being very susceptible, and a "9" beingvery resistant.*

PCT TILLER

The total number of tillers per plot divided by the total number ofplants per plot.

PLANT

This term includes plant cells, plant protoplasts, plant cell tissuecultures from which corn plants can be regenerated, plant calli, plantclumps, and plant cells that are intact in plants or parts of plants,such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks,stalks, roots, root tips, anthers, silk and the like.

PLANT HEIGHT

The distance in centimeters from ground level to the base of the tasselpeduncle.

RM

Predicted relative maturity based on the moisture percentage of thegrain at harvest. This rating is based on known set of checks andutilizes standard linear regression analyses and is referred to as theMinnesota Relative Maturity Rating System.

SHED

The volume of pollen shed by the male flower rated on a 1-9 scale wherea "1" is a very light pollen shedder, a "4.5" is a moderate shedder, anda "9" is a very heavy shedder. If the Table(s) 3 have reduced the 1-9shed scale to a 1-3 shed scale then any shed Table 3 can be multipliedby 3 to reach the 1-9 shed scale.

SLB

Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated ona 1-9 scale with a "1" being very susceptible, and a "9" being veryresistant.*

TWT

The measure of the weight of grain in pounds for a one bushel volumeadjusted for percent grain moisture.

VIGOR

Visual rating of 1 to 9 made 2-3 weeks post-emergence where a "1 "indicates very poor early plant development, and a "9" indicatessuperior plant development.

WARM GERM

A measurement of seed germination under ideal (warm, moist) conditions.Data is reported as percent of seeds germinating.

YIELD (YLD)

Actual yield of grain at harvest adjusted to 15.5% moisture.Measurements are reported in bushels per acre.

% DROPPED EARS (DE)

The number of plants per plot which dropped their primary ear divided bythe total number of plants per plot.

% LRG FLAT

Percentage by weight of shelled corn that passes through a 26/64 inchround screen and a 14/64 inch slot screen, but does not pass through ascreen with 20.5/64 inch round openings.

% LRG ROUND

Percentage by weight of shelled corn that passes through a 26/64 inchround screen, but does not pass through a 14/64 inch slot screen or ascreen with 20.5/64 inch round openings.

% MED FLAT

Percentage by weight of shelled corn that passes through a 20.5/64 inchround screen and a 13/64 inch slotted screen, but does not pass througha screen with 17/64 inch round openings.

% MED ROUND

Percentage by weight of shelled corn that passes through a 20.5/64 inchround screen, but does not pass through a 13/64 inch slot screen or ascreen with 17/64 inch round openings.

% SML FLAT

Percentage by weight of shelled corn that passes through a 17/64 inchround screen and a 12/64 inch slotted screen, but does not pass througha screen with 15/64 inch round openings.

%SML ROUND

Percentage by weight of shelled corn that passes through a 17/64 inchround screen, but does not pass through a 12/64 inch slotted screen or ascreen with 15/64 inch round openings.

% ROOT LODGE (RL)

Percentage of plants per plot leaning more that 30 degrees from verticaldivided by total plants per plot.

% STALK LODGE (SL)

Percentage of plants per plot with the stalk broken below the primaryear node divided by the total plants per plot.

* Resistant--on a scale of 1-9 with 9 evidencing the trait moststrongly: 1-2.9 ratings are susceptible, 3-5.9 ratings are intermediate,and 6-9 ratings are resistant.

DETAILED DESCRIPTION OF THE INVENTION

ZS01602 can be used as a female or a male line due to its pollen shedand seed production abilities. This ZS01602 line evidences good generalcombining ability and specific combining ability especially withLancasters. This line evidences good stability and heat stresstolerances.

The inbred has shown uniformity and stability within the limits ofenvironmental influence for all the traits as described in the VarietyDescription Information (Table 1) that follows. Most of the data in theVariety Description information was collected at Slater, Iowa.

The inbred has been self-pollinated for a sufficient number ofgenerations to give inbred uniformity. During plant selection in eachgeneration, the uniformity of plant type was selected to ensurehomozygosity and phenotypic stability. The line has been increased inisolated farmland environments with data on uniformity and agronomictraits being observed to assure uniformity and stability. No varianttraits have been observed or are expected in ZS01602.

The best method of producing the invention, ZS01602 which issubstantially homozygous, is by planting the seed of ZS01602 which issubstantially homozygous and self-pollinating or sib pollinating theresultant plant in an isolated environment, and harvesting the resultantseed or the resultant pollen.

                  TABLE 1                                                         ______________________________________                                        ZS01602                                                                       VARIETY DESCRIPTION INFORMATION                                               ______________________________________                                        #1      Type: Dent                                                            #2      Region Best Adapted: South and southeastern regions                   ______________________________________                                    

    __________________________________________________________________________    1995 PROJECT MO PVP TRAITS                                                    INBRED ZS01602                                                                __________________________________________________________________________                          N  MEAN                                                                              STD.                                                                              T-STAT                                                                             PROB                                                                               95%  CI                            __________________________________________________________________________    EAR HEIGHT (CM)       15 53.33                                                                             4.58                                                                              45.13                                                                              0.0000                                                                            (51.02, 55.65)                      LENGTH OF PRIMARY EAR LEAF (CM)                                                                     15 87.12                                                                             2.54                                                                              133.0                                                                              0.0000                                                                            (85.84, 88.40)                      WIDTH OF PRIMARY EAR LEAF (CM)                                                                      15 9.41                                                                              0.44                                                                              82.51                                                                              0.0000                                                                            (9.18, 9.63)                        TOP EAR INTERNODE (CM)                                                                              15 12.50                                                                             1.18                                                                              41.02                                                                              0.0000                                                                            (11.90, 13.10)                      DEGREE OF LEAF ANGLE  15 12.80                                                                             2.01                                                                              24.70                                                                              0.0000                                                                            (11.78, 13.82)                      # OF EARS PER PLANT   15 1.33                                                                              0.49                                                                              10.58                                                                              0.0000                                                                            (1.09, 1.58)                        # OF LEAVES ABOVE TOP EAR                                                                           15 5.67                                                                              0.49                                                                              44.98                                                                              0.0000                                                                            (5.42, 5.91)                        # OF PRIMARY LATERAL TASSEL BRANCHES                                                                15 4.53                                                                              0.83                                                                              21.06                                                                              0.0000                                                                            (4.11, 4.96)                        PLANT HEIGHT (CM)     15 142.9                                                                             10.94                                                                             50.58                                                                              0.0000                                                                            (137.3, 148.4)                      TASSEL LENGTH (CM)    15 44.87                                                                             1.81                                                                              96.14                                                                              0.0000                                                                            (43.95, 45.78)                      TASSEL BRANCH ANGLE   15 10.47                                                                             4.79                                                                              8.46 0.0000                                                                             (8.04, 12.89)                      # OF TILLER PER PLANTS                                                                              15 0.27                                                                              0.46                                                                              2.26 0.0406                                                                            (0.04, 0.50)                        WEIGHT PER 100 KERNELS (GM)                                                                         15 22.97                                                                             2.70                                                                              32.97                                                                              0.0000                                                                            (21.61, 24.34)                      EAR LENGTH (CM)       15 14.83                                                                             1.23                                                                              46.54                                                                              0.0000                                                                            (14.21, 15.46)                      EAR WEIGHT (GM)       15 113.0                                                                             15.48                                                                             28.27                                                                              0.0000                                                                            (105.2, 120.8)                      # OF KERNEL ROWS      15 17.20                                                                             2.70                                                                              24.63                                                                              0.0000                                                                            (15.83, 18.57)                      COB DIAMETER AT MID-POINT (MM)                                                                      15 25.13                                                                             0.74                                                                              131.0                                                                              0.0000                                                                            (24.76, 25.51)                      EAR DIAMETER AT MID-POINT (MM)                                                                      15 41.27                                                                             1.39                                                                              115.2                                                                              0.0000                                                                            (40.56, 41.97)                      KERNEL LENGTH (MM)    15 9.60                                                                              0.83                                                                              44.90                                                                              0.0000                                                                             (9.18, 10.02)                      KERNEL THICKNESS (MM) 15 5.27                                                                              0.88                                                                              23.08                                                                              0.0000                                                                            (4.82, 5.71)                        KERNEL WIDTH (MM)     15 7.33                                                                              0.82                                                                              34.79                                                                              0.0000                                                                            (6.92, 7.75)                        % ROUND KERNELS (SHAPE GRADE)                                                                       15 32.80                                                                             12.51                                                                             10.16                                                                              0.0000                                                                            (26.47, 39.13)                      SHANK LENGTH          15 11.20                                                                             1.61                                                                              26.90                                                                              0.0000                                                                            (10.38, 12.02)                      __________________________________________________________________________    INBRED                                                                             MO:ZS01602                                                               __________________________________________________________________________    #3 MATURITY                                                                        HEAT                                                                     DAYS UNITS                                                                    63   1534 FROM PLANTING TO 50% OF PLANTS IN SILK                              61   1481 FROM PLANTING TO 50% OF PLANTS IN POLLEN                             9        FROM 10% TO 90% POLLEN SHED                                         #4 PLANT                                                                      DATA                                                                           3        ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3 = MODERATE 4               = DARK                                                              #5 LEAF                                                                       COLOR/DATA                                                                     3/DARK GREEN                                                                           LEAF COLOR **MUNSELL CODE-5GY 4/4                                    3        LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH FUZZ)                  5        MARGINAL WAVES (1 = NONE TO 9 = MANY)                                3        LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)                         #6 TASSEL                                                                     COLOR/DATA                                                                     4        POLLEN SHED (0 = STERILE TO 9 = HEAVY SHEDDER)                       6/PALE YELLOW                                                                          ANTHER COLOR **MUNSELL CODE-5Y 8/9                                   2/MEDIUM GREEN                                                                         GLUME COLOR **MUNSELL CODE-5GY 5/8 W/S 2.5R 4/8                      1        BAR GLUME: 1 = ABSENT 2 = PRESENT                                   #7A EAR (HUSKED DATA)                                                         COLOR/DATA                                                                     6/PALE YELLOW                                                                          SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-5Y 8/6               2/MEDIUM GREEN                                                                         FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL CODE-5GY 6/6          21/BUFF   DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL CODE-2.5Y 8/3       1        POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2 = HORIZONTAL 3 =                   PENDENT                                                              5        HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT)                    4        HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8 CM 3 = 8-10 CM               4 => 10 CM                                                          #7B EAR (HUSKED DATA)                                                         DATA                                                                           2        KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT                             1        ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 = SPIRAL                1        EAR TAPPER: 1 = STRAIT 2 = AVERAGE 3 = EXTREME                      #8 KERNEL (DRY)                                                               COLOR/DATA                                                                     1        ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG                             7/YELLOW ALEURONE COLOR **MUNSELL CODE-2.5Y 8/10                              7/YELLOW HARD ENDOSPERM COLOR **MUNSELL CODE-2.5Y 7/10                        3        ENDOSPERM TYPE                                                       7/YELLOW CROWN COLOR **MUNSELL CODE-2.5Y 8/10                                #9 COB                                                                        COLOR                                                                         12/LIGHT RED                                                                            COB COLOR **MUNSELL CODE-10R 6/6                                    #11 DISEASE RESISTANCE                                                        Northern leaf blight = 4.5                                                    NLSr2* = 5.5                                                                  Gray leaf spot = 4.5                                                          Eye = 5                                                                       GW = 6.5                                                                      MDMVB = 1                                                                     __________________________________________________________________________     * Northern Leaf Spot Race Two                                            

The genetically related line to ZS01602 is Holden's Inbred #117 . Otherlines which are comparable in usage or maturity or genetically are B73and ZS0441 PVP-0200038.

The Munsell code is a reference book of color which is known and used inthe industry and by persons with ordinary skill in the art of plantbreeding.

The purity and homozygosity of inbred ZS01602 is constantly beingtracked using isozyme genotypes as shown in Table 2.

Isozyme Genotypes for ZS01602

Isozyme data were generated for inbred corn line ZS01602 according toprocedures known and published in the art. The data in Table 2 gives theelectrophoresis data on ZS01602.

                                      TABLE 2                                     __________________________________________________________________________    ELECTROPHORESIS RESULTS FOR ZS01602                                           __________________________________________________________________________    INBRED                                                                             ACP1                                                                              ACP4                                                                              ADH                                                                              MDH1                                                                              MDH2                                                                              PGD1                                                                              PGD2                                                                              PH1                                                                              PGM                                                                              IDH2                                    __________________________________________________________________________    ZS01602                                                                            11  22  22 22  11  11  11  22 22 22                                      __________________________________________________________________________

Inbred and Hybrid Performance of ZS01602

The traits and characteristics of inbred corn line ZS01602 are listed tocompare with other inbreds and/or in hybrid combination. ZS01602 datashows the characteristics and traits of importance, giving a snapshot ofZS01602.

Table 3A shows a comparison between ZS01602 and B73. ZS01602 hassignificantly lower ear placement than does B73. ZS01602 has higheryield and lower grain moisture at harvest than does B73. ZS01602 flowersslightly earlier than B73 across all pollination and silking data.ZS01602 reaches heat peek at the same number of heat units than doesB73. ZS01602 has slightly higher germination under cold testingconditions than does B73.

                                      TABLE 3A                                    __________________________________________________________________________    PAIRED INBRED COMPARISON DATA                                                 __________________________________________________________________________                          PCT  PLANT                                                                              EAR         EAR   PCT                         YEAR  INBRED                                                                             VIGOR EMERGE                                                                             TILLER                                                                             HEIGHT                                                                             HEIGHT                                                                             SHED   QUALITY                                                                             BARREN                      __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            7.0   92.5      172.1                                                                              79.4 7.8                                            B73  6.1   86.7      180.0                                                                              100  8.0                                            # EXPTS                                                                            4     4         4    4    4                                              DIFF 0.9   5.8       7.9  20.6 0.3                                            PROB 0.133 0.230     0.157                                                                              0.064***                                                                           0.638                                    __________________________________________________________________________    YEAR  INBRED                                                                             HEATP10                                                                             HEATP50                                                                            HEATP90                                                                            HEATS10                                                                            HEATS50                                                                            HEATS90                                  __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1530  1558 1627 1564 1581 1618                                           B73  1551  1591 1668 1592 1626 1661                                           # EXPTS                                                                            4     4    4    4    4    4                                              DIFF 21    33   40   28   44   43                                             PROB 0.319 0.257                                                                              0.186                                                                              0.253                                                                              0.155                                                                              0.157                                    __________________________________________________________________________                          BL   % ROOT                                                                             % STALK                                                                            % DROPPED                                YEAR  INBRED                                                                             HEATPEEK                                                                            HEATBL                                                                             MOIST                                                                              LODGE                                                                              LODGE                                                                              EARS   MOISTURE                                                                            YIELD                       __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1496  2734 31.0                  11.9  110.4                             B73  1496  2652 29.5                  13.5  99.0                              # EXPTS                                                                            4     1    1                     4     4                                 DIFF 0     82   1.5                   1.5   11.4                              PROB 0.995                            0.009*                            __________________________________________________________________________               WARM  COLD % LRG                                                                              % LRG                                                                              % MED                                                                              % MED  % SML % SML                       YEAR  INBRED                                                                             GERM  GERM ROUND                                                                              FLAT ROUND                                                                              FLAT   ROUND FLAT                        __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            96.6  92.0 11.6 13.1 25.2 36.2   5.0   6.7                               B73  97.9  89.1 5.5  6.0  28.2 43.0   7.0   8.8                               # EXPTS                                                                            4     4    4    4    4    4      4     4                                 DIFF 1.3   2.9  6.1  7.1  3.0  6.8    2.1   2.2                               PROB 0.231 0.575                                                                              0.036**                                                                            0.006*                                                                             0.372                                         __________________________________________________________________________

Table 3B compares ZS01602 with Holden's Inbred #117. ZS01602 has betteryield by 22 bushels and has lower grain moisture at harvest than doesHolden's Inbred #117 . ZS01602 has slightly more seedling vigor and is asignificantly taller plant with lower ear placement than is inbredHolden's Inbred #117. ZS01602 has slightly higher cold germinationresults in testing than does Holden's Inbred #117. ZS01602 flower atsignificantly lower numbers of heat units across all silking andpollination data.

                                      TABLE 3B                                    __________________________________________________________________________    PAIRED INBRED COMPARISON DATA                                                 __________________________________________________________________________                          PCT  PLANT                                                                              EAR         EAR   PCT                         YEAR  INBRED                                                                             VIGOR EMERGE                                                                             TILLER                                                                             HEIGHT                                                                             HEIGHT                                                                             SHED   QUALITY                                                                             BARREN                      __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            7.0   92.5      172.1                                                                              79.4 7.8                                            Holden's                                                                           6.5   89.7      156.5                                                                              82.5 8.0                                            Inbred                                                                        #117                                                                          # EXPTS                                                                            4     4         4    4    4                                              DIFF 0.5   2.8       15.6 3.2  0.3                                            PROB 0.423 0.408     0.029**                                                                            0.564                                                                              0.391                                    __________________________________________________________________________    YEAR  INBRED                                                                             HEATP10                                                                             HEATP50                                                                            HEATP90                                                                            HEATS10                                                                            HEATS50                                                                            HEATS90                                  __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1530  1558 1627 1564 1581 1618                                           Holden's                                                                           1625  1668 1747 1655 1695 1754                                           Inbred                                                                        #117                                                                          # EXPTS                                                                            4     4    4    4    4    4                                              DIFF 96    109  120  91   113  136                                            PROB 0.014**                                                                             0.003*                                                                             0.004*                                                                             0.011**                                                                            0.011**                                                                            0.000*                                   __________________________________________________________________________                          BL   % ROOT                                                                             % STALK                                                                            % DROPPED                                YEAR  INBRED                                                                             HEATPEEK                                                                            HEATBL                                                                             MOIST                                                                              LODGE                                                                              LODGE                                                                              EARS   MOISTURE                                                                            YIELD                       __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1496  2734 31.0                  11.9  110.4                             Holden's                                                                           1537  2702 32.5                  13.5  87.6                              Inbred                                                                        #117                                                                          # EXPTS                                                                            4     1    1                     4     4                                 DIFF 40    32   1.5                   1.5   22.8                              PROB 0.113                            0.179                             __________________________________________________________________________               WARM  COLD % LRG                                                                              % LRG                                                                              % MED                                                                              % MED  % SML % SML                       YEAR  INBRED                                                                             GERM  GERM ROUND                                                                              FLAT ROUND                                                                              FLAT   ROUND FLAT                        __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            96.6  92.0 11.6 13.1 25.2 36.2   5.0   6.7                               Holden's                                                                           96.2  84.0 5.1  7.2  32.4 40.7   7.5   5.2                               Inbred                                                                        #117                                                                          # EXPTS                                                                            4     4    4    4    4    4      4     4                                 DIFF 0.4   8.0  6.5  5.9  7.2  4.5    2.5   1.5                               PROB 0.536 0.227                                                                              0.002*                                                                             0.055***                                                                           0.184                                         __________________________________________________________________________

Table 3C is an inbred comparison between ZS01602 and ZS0441. ZS01602 hassignificantly better pollen shed than does ZS0441 as well as havingbetter emergence of plants. ZS01602 is significantly later to reachHeatS 10 and HeatS50 than is ZS0441. ZS01602 reaches both HeatPEEK andHeatBL significantly later than ZS0441. ZS01602 reaches all three ratedlevels of pollination (HeatP10-90) approximately the same time asZS0441. The two inbreds are the same in yield and moisture.

                                      TABLE 3C                                    __________________________________________________________________________    PAIRED INBRED COMPARISON DATA                                                 __________________________________________________________________________                          PCT  PLANT                                                                              EAR         EAR   PCT                         YEAR  INBRED                                                                             VIGOR EMERGE                                                                             TILLER                                                                             HEIGHT                                                                             HEIGHT                                                                             SHED   QUALITY                                                                             BARREN                      __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            6.5   87.7      167.3                                                                              69.1 6.8                                            ZS0441                                                                             6.5   85.7      163.9                                                                              68.7 5.9                                            # EXPTS                                                                            15    15        15   15   13                                             DIFF 0.1   2.0       3.4  0.4  0.9                                            PROB 0.862 0.497     0.156                                                                              0.857                                                                              0.008*                                   __________________________________________________________________________    YEAR  INBRED                                                                             HEATP10                                                                             HEATP50                                                                            HEATP90                                                                            HEATS10                                                                            HEATS50                                                                            HEATS90                                  __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1466  1512 1619 1532 1566 1613                                           ZS0441                                                                             1462  1512 1634 1475 1530 1596                                           # EXPTS                                                                            13    13   13   13   13   13                                             DIFF 4     0    14   57   36   17                                             PROB 0.709 0.993                                                                              0.301                                                                              0.001*                                                                             0.024**                                                                            0.379                                    __________________________________________________________________________                          BL   % ROOT                                                                             % STALK                                                                            % DROPPED                                YEAR  INBRED                                                                             HEATPEEK                                                                            HEATBL                                                                             MOIST                                                                              LODGE                                                                              LODGE                                                                              EARS   MOISTURE                                                                            YIELD                       __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            1431  2673 31.0                  12.9  78.8                              ZS0441                                                                             1383  2636 27.5                  12.9  79.1                              # EXPTS                                                                            13    3    1                     15    15                                DIFF 48    36   3.5                   0.0   0.4                               PROB 0.000*                                                                              0.050**                    0.928                             __________________________________________________________________________               WARM  COLD % LRG                                                                              % LRG                                                                              % MED                                                                              % MED  % SML % SML                       YEAR  INBRED                                                                             GERM  GERM ROUND                                                                              FLAT ROUND                                                                              FLAT   ROUND FLAT                        __________________________________________________________________________    OVERALL                                                                             ZS01602                                                                            95.2  85.2 9.9  10.0 27.0 36.7   6.9   7.7                               ZS0441                                                                             94.5  80.8 14.7 27.9 24.0 28.4   2.9   1.2                               # EXPTS                                                                            10    10   13   13   13   13     13    13                                DIFF 0.6   4.4  4.8  18.0 2.9  8.3    4.1   6.5                               PROB 0.500 0.172                                                                              0.024**                                                                            0.000*                                                                             0.139                                         __________________________________________________________________________

Table 4A shows the GCA (general combining ability) estimates of ZS01602compared with the GCA estimates of the other inbreds. The estimates showthe general combining ability is weighted by the number ofexperiment/location combinations in which the specific hybridcombination occurs. The interpretation of the data for all traits isthat a positive comparison is a practical advantage. A negativecomparison is a practical disadvantage. The general combining ability ofan inbred is clearly evidenced by the results of the general combiningability estimates. This data compares the inbred parent in a number ofhybrid combinations to a group of "checks". The check data is from othercompanies' hybrids, particularly the leader in the industry and ICISeeds' commercial products and pre-commercial hybrids which were grownin the same sets and locations.

Table 4A shows ZS01602 crossed to 308 different inbreds. ZS01602 inhybrid combination shows an advantage for yield. ZS01602 has a slightdisadvantage for moisture at -1.5. ZS01602 has less tendency to havestalk lodging and root lodging or dropped ears than the checks.

ZS01602 in random hybrid combinations has the GCA shown in Table 4A.When only hybrids containing Lancaster material are analyzed, it becomesclear that ZS01602 has specific combining ability with this type ofheterotic pattern of maize.

                                      TABLE 4A                                    __________________________________________________________________________    GCA & SCA ESTIMATES                                                           ADVANTAGES OVER COMMERCIALS & N'S                                             ENT     N93                                                                              N94                                                                              N95                                                                              N  FI V M                                                                              GI                                                                              I VLD                                                                              MST                                                                              % SL                                                                             % RL                                                                             % DE                                                                              TWT                                                                              POP                                                                              RM                        __________________________________________________________________________    ZS01602                                                                            XR =                                                                             222                                                                              8  73 308                                                                              -0.8                                                                             -0.3                                                                             2.6                                                                             3.1                                                                             3.9                                                                              -1.5                                                                             0.6                                                                              0.1                                                                              0.0 -0.6                                                                               14                                                                             124                            XH =         39                                                                              -1.6                                                                             -0.4                                                                             1.8                                                                             3.5                                                                             2.9                                                                              -1.5                                                                             0.3                                                                              0.0                                                                              0.0 -0.7                                                                               19                                                                             124                            XT =         6 0.9                                                                              -0.0                                                                             3.4                                                                             4.1                                                                             6.2                                                                              -1.1                                                                             0.3                                                                              0.1                                                                              0.0 -0.4                                                                             -52                                                                              124                       __________________________________________________________________________     XR = GCA ESTIMATE: WEIGHTED BY EXPT                                           XH = GCA ESTIMATE: WEIGHTED BY PARENT2                                        XT = SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA     

Table 5 shows the yield response of ZS01602 in hybrid combination incomparison with the plants in the environment around it at the samelocation. ZS01602 in hybrid combination yields well across all yieldlevels. This type of stability of yield appears throughout years oftesting in different environments.

                  TABLE 5                                                         ______________________________________                                        YIELD RESPONSE                                                                HYBRID         YIELD                                                          ______________________________________                                        ZS01602/Inbred                                                                          78      104    130    157  182    209                               Environment                                                                             75      100    125    150  175    200                               ______________________________________                                    

Table 6A shows in positive numbers the advantage the ZS01602 hybrid hasover two commercially available ICI Seeds' hybrids. ZS01602 hybridcompares favorably with both 8344 and 8315. ZS01602 in hybrid comparisonhas better yield results and less dropped ears and better stalks thaneither commercial line. ZS01602 in hybrid combination has a better Gindex rating than either commercial hybrid.

Additionally, ZS01602 has a slight disadvantage in moisture over the twocommercials. However, the overall ZS01602 hybrid carries a strongpackage of agronomic traits.

                                      TABLE 6A                                    __________________________________________________________________________    HYBRID SUMMARY                                                                ZS01602/INBRED                                                                PERFORMANCE DATA                                                              __________________________________________________________________________                   CORN PAIRED COMPARISONS                                        HYBRID YEAR                                                                              TESTS                                                                             GI ADV                                                                              FI                                                                              ADV                                                                              YLD                                                                              ADV                                                                              MOIST                                                                             ADV                                                                              SL ADV                                 __________________________________________________________________________    ZS01602                                                                            RE    5   145                                                                               7 97                                                                               5 102.9                                                                            3.3                                                                              20.9                                                                              -0.7                                                                              6.0                                                                             4.6                                 8344 RE    5   138   92    99.6 20.2   10.5                                        RE    5   145                                                                               7 97                                                                               5 102.9                                                                            3.3                                                                              20.9                                                                              -0.7                                                                              6.0                                                                             4.6                                      RE    5   138   92    99.6 20.2   10.5                                   ZS01602                                                                            RE    5   145                                                                              13 97                                                                              12 102.9                                                                            8.6                                                                              20.9                                                                              -0.4                                                                              6.0                                                                             9.0                                 8315 RE    5   132   85    94.3 20.5   14.9                                        RE    5   145                                                                              13 97                                                                              12 102.9                                                                            8.6                                                                              20.9                                                                              -0.4                                                                              6.0                                                                             9.0                                      RE    5   132   85    94.3 20.5   14.9                                   __________________________________________________________________________                   CORN PAIRED COMPARISONS                                        HYBRID YEAR                                                                              TESTS                                                                             RL ADV                                                                              DE  ADV                                                                              TWT  ADV                                                                              POP   ADV                                 __________________________________________________________________________    ZS01602                                                                            RE    5   1.6                                                                                0.9                                                                            0.0 0.0        24385 209                                 8344 RE    5   2.5   0.0            24176                                          RE    5   1.6                                                                                0.9                                                                            0.0 0.0        24385 209                                      RE    5   2.5   0.0            24176                                     ZS01602                                                                            RE    5   1.6                                                                              -0.2                                                                             0.0 0.2        24385  -9                                 8315 RE    5   1.4   0.2            24394                                          RE    5   1.6                                                                              -0.2                                                                             0.0 0.2        24385  -9                                      RE    5   1.4   0.2            24394                                     __________________________________________________________________________

Table 7A shows the entomology strengths and weakness of ZS01602 as aninbred.

                  TABLE 7A                                                        ______________________________________                                                                    EAR    PLANT                                              ECB1     ECB2       DAMAGE INTEGRITY                                  INBRED  RATING   RATING     RATING RATING                                     ______________________________________                                        ZS01602 5.8      5.7        1.5    6.3                                        ______________________________________                                    

The inbred ZS01602 can be employed as the female or the male plant in ahybrid production field. The female has good seed size and abundantseed. The mail is an excellent pollen shedder. Additionally, this inbredcarries heat stress tolerance and good disease resistance.

In hybrid combination, ZS01602 carries good yield stability overenvironments into the hybrid. ZS01602 shows good performance in heat andstress and tends to carry this trait into its hybrids. This inbredadapts well across the Southern region when in hybrid combination. Thisinbred has fairly good general combining and specific combining abilitywith Lancasters for yield with most lines. ZS01602 is a versatile line.

The foregoing is set forth by way of example and is not intended tolimit the scope of the invention.

This invention also is directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plantwherein the first or second parent corn plant is an inbred corn plantfrom the line ZS01602. Further, both first and second parent corn plantscan come from the inbred corn line ZS01602. A variety of breedingmethods can be selected depending on the mode of reproduction, thetrait, the condition of the germplasm. Thus, any such methods using theinbred corn line ZS01602 are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, haploid andanther culturing and the like.

Various culturing techniques known to those skilled in the art, such ashaploid, transformation, and a host of other conventional andunconventional methods are within the scope of the invention. All plantsand plant cells produced using inbred corn line ZS01602 are within thescope of this invention. The invention encompasses the inbred corn lineused in crosses with other, different, corn inbreds to produce (F1) cornhybrid seeds and plants with the characteristics that make good hybrids.This invention includes cells which upon growth and differentiationproduce corn plants having the physiological and morphologicalcharacteristics of the inbred line ZS01602.

Duncan, from at least 1985-1988 produced literature on plantregeneration from callus. Both inbred and hybrid callus have resulted inregenerated plants at excellent efficiency rates. Somatic embryogenesishas been performed on various maize tissue such as glume which beforethe 1980's was considered unusable for this purpose. The prior artclearly teaches the regeneration of plants from various maize tissues.

European Patent Application, publication 160,390, describes tissueculture of corn which can be used by those skilled in the art. Corntissue culture procedures are also described in the literature as earlyas 1982.

A deposit of at least 2500 seeds of the inbred seed of this invention ismaintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Accessto this deposit will be available during the pendency of thisapplication to the Commissioner of Patents and Trademarks and personsdetermined by the Commissioner to be entitled thereto upon request. TheApplicant made a deposit one Jun.11, 1997, of at least 2500 seeds ofInbred Corn Line ZS01602 with the American Type Culture Collection(ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCCAccession number is 209116. Additionally, the Applicant has satisfiedall of the requirements of C.F.R. 1.801-1.809, including providing anindication of the viability of the sample. The ATCC deposit will bemaintained in that depository, which is a public depository, for aperiod of 30 years, or 5 years after the last request, or for theeffective life of the patent, whichever is longer, and will be replacedif it becomes nonviable during that period.

Inbreds designated MBS are available from Mike Brayton Seed in Iowa.Inbreds designated SGI are available from Seed Genetic Inc. in NewJersey. Information on some ZS designations may be available from thePVP office.

Accordingly, the present invention has been described with some degreeof particularity directed to the preferred embodiment of the presentinvention. It should be appreciated, though, that the present inventionis defined by the following claims construed in light of the prior artso that modifications or changes may be made to the preferred embodimentof the present invention without departing from the inventive conceptscontained herein.

We claim:
 1. Inbred corn seed designated ZS01602, some of said seeddeposited in the ATCC and carry accession number
 209116. 2. A corn plantproduced by the seed of claim
 1. 3. A tissue culture of regenerablecells of ZS01602 of claim 1 wherein the cells of the tissue cultureregenerates plants comprising the phenotype of ZS01602.
 4. A tissueculture according to claim 3, the tissue culture selected from the groupconsisting of leaves, pollen, embryos, roots, root tips, anthers, silk,flowers, kernels, ears, cobs, husks and stalks, and cells andprotoplasts thereof.
 5. A corn plant comprising the phenotype of ZS01602regenerated from the cells of the tissue culture of claim
 3. 6. Hybridseed produced by:(a) planting, in pollinating proximity, seeds accordingto claim 1 of corn inbred lines ZS01602 and another inbred line, one ofsaid inbred lines not releasing pollen; (b) cultivating corn plantsresulting from said planting; (c) allowing natural cross pollinating tooccur between said inbred lines; and (d) harvesting seeds produced onthe non pollen releasing inbred.
 7. Hybrid seed produced by hybridcombination of plants of inbred corn seed designated ZS01602 in claim 1and plants of another inbred line.
 8. Hybrid plants grown from seed ofclaim
 7. 9. A first generation (F1) hybrid corn plant produced by usingZS01602 according to claim 1 the process of:(a) planting, in pollinatingproximity, seeds of corn inbred lines ZS01602 and another inbred line;(b) cultivating corn plants resulting from said planting; (c) preventingpollen production by the plants of one of the inbred lines; (d) allowingnatural cross pollinating to occur between said inbred lines; (e)harvesting seeds produced on plants of the inbred line of step (c); and(f) growing a harvested seed of step (e).
 10. A tissue culture of theregenerable cells of the corn plant of claim
 8. 11. A tissue culture ofthe regenerable cells of the corn plant of claim
 9. 12. An inbred cornplant with all the phenotypic, physiological and morphologicalcharacteristics of inbred corn line of claim 2.